Use of hr 3 enhancer and P 74 TM domain in baculovirus surface display
نویسندگان
چکیده
1 The Key Laboratory of Cell Proliferation and Differentiation of Ministry of Education and The State Key Laboratory of Biomembrane and Membrane Bio-engineering, College of Life Sciences, Peking University, Beijing 100871, P. R. China, The Center for Theoretical Biology, Peking University, Beijing 100871, P. R. China, Institute of Virology, Zhejiang Provincial Center For Disease Prevention and Control, Hangzhou, Zhejiang 310009, P. R. China, 4 Neuroscience Research Institute and Infectious Disease Center, Health Science Center, Peking University, Beijing 100191, P.R.China
منابع مشابه
The highly conserved basic domain I of baculovirus IE1 is required for hr enhancer DNA binding and hr-dependent transactivation.
The immediate-early protein IE1 is the principal transcriptional regulator of the baculovirus Autographa californica nucleopolyhedrovirus (AcMNPV). Transactivation by IE1 is dramatically stimulated by cis linkage of the affected promoter to AcMNPV homologous region (hr) elements that contain palindromic 28-bp repeats (28-mers) with enhancer activity. This hr-dependent transcriptional enhancemen...
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متن کاملNovel baculovirus DNA elements strongly stimulate activities of exogenous and endogenous promoters.
A DNA sequence upstream from the polyhedrin gene of baculovirus Autographa californica nucleopolyhedrovirus (AcMNPV) was found to activate strongly the expression of full or minimal promoters derived from AcMNPV and other sources. Promoters tested included the minimal CMV (CMVm) promoter from human cytomegalovirus, the full heat shock 70 promoter from Drosophila, and the minimal p35 promoter fr...
متن کاملBaculovirus transregulator IE1 requires a dimeric nuclear localization element for nuclear import and promoter activation.
Immediate-early protein IE1 is a principal regulator of viral transcription and a contributor to origin-specific DNA replication of the baculovirus Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV). Since these viral functions involve interaction of dimeric IE1 with palindromic homologous region (hr) enhancer-origin elements of the AcMNPV genome within the nucleus, it is presumed...
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تاریخ انتشار 2011